ࡱ>  bjbjww .^k^kEH\ \ UR<8/#$(---"""""""%(d""--#--""-0`"#0#$(@X(( ""d#$(\ : Multiplexed TrAEL-seq The multiplexed protocol and adaptors work so well we rarely if ever use the original protocol now. Reagents and consumables are detailed after the protocol Common problems and solutions are given at the end of this document Input material Start with agarose plugs containing 1-4x10^7 yeast cells that were ethanol fixed at harvest or 1-2x10^6 mammalian cells (see protocol for Agarose Embedding), you need of a plug per sample. Stopping points This protocol is very long but can be done in bits, and we have stopped at many points without problems. To our knowledge all the following stopping points are fine: You can pause indefinitely at any point where plugs are in tris buffer, store at 4 You can pause indefinitely when DNA is in solution in TE, store at 4. You can pause indefinitely when DNA solutions have ethanol added for precipitation, is a pellet in the 70% ethanol, or the pellet is re-suspending in TE. Store at 4. You can pause indefinitely when DNA is bound to magnetic beads once beads are in bead wash or TE, store at 4. Vortex well to re-suspend when you re-start. Once you start the library preps using the NEBNext Ultra II kit, you must continue until the beads are in the wash solution, but then you can store at 4. After eluting the DNA from the magnetic beads with USER, store the libraries in the freezer until ready to amplify. Similarly, amplified libraries before or after purification are stored in the freezer. The latest version of this protocol should be available from our lab website: HYPERLINK "/our-research/epigenetics/jon-houseley/protocols"/our-research/epigenetics/jon-houseley/protocols Protocol NOTE: At the start you are handling each sample separately, they are pooled later Transfer plug 2ml tube Equilibrate plug once in 100l 1x TdT buffer, 30 min at room temperature (RT) Exchange for 100ul containing: 10ul 10x Terminal transferase buffer (NEB) 0.4ul 100mM ATP 1ul Terminal transferase (NEB) 84ul water Incubate 2-3 hrs at 37 Discard the solution and rinse plug with 1ml tris buffer Then equilibrate plug 1 hr at RT with: 10ul 10x T4 RNA ligase buffer (NEB) 40ul 50% PEG 8000 50ul water Exchange for 100ul containing: 10ul 10x T4 RNA ligase buffer 40ul 50% PEG 8000 1ul App TrAEL indexed adaptor (indexes 1,3,5,6,7 or 8) 48ul water 1ul T4 RNA ligase 2 KQ Mix all components except ligase in a 1.5ml tube, vortex vigorously, then add ligase and pipette repeatedly with a P200 cut tip to mix it really well Incubate overnight at 25deg [NOT LONGER!!!] Discard reaction mix, rinse plug 1x 1ml tris buffer, transfer to 15ml tube During the day wash 4x with 10ml tris buffer at room temperature on rocker Leave overnight on rocker with 10ml tris buffer Equilibrate 15min or more with 1ml agarase buffer Meanwhile, heat a block to 65 and another to 42C  Now pool the libraries: Transfer all the plugs to a single 1.5 ml tube (up to 6 plugs with different indexes) This is a bit fiddly. The easiest way we have found is to pour the 1ml agarase buffer + plug from the 15ml falcon tube into an intermediate 1.5ml tube and remove the buffer so that you have the plug by itself. Then, invert this over the tube in which you collecting the plugs and flick gently until it falls down into the tube with the other plugs Remove any residual agarase buffer, add agarase buffer if necessary to a final volume of ~300 l and melt 20 min at 65 Shift tubes to 42 for 5 min, add 1ul -agarase per plug, flick, incubate 1 hour at 42 Be quick during addition  do not let the tube cool down Add 75 l 10M NH4OAc, 3 l glycoblue and 1ml of ethanol, vortex vigorously (particularly for mammalian samples) Chill 15 min on ice then spin 15 min top speed 4  Wash with 70% ethanol, dry 5 min, resuspend in 10l 0.1xTE per plug, leave >10 min at room temperature For mammalian samples 15 min at 65 is better but make sure it doesnt go dry Per plug, add master mix containing 29l water 5l Isothermal amp buffer (NEB) 3l 100mM MgSO4 2l dNTPs (10mM) 1l Bst 2 warmstart pol (NEB) Pipette to mix and re-suspend pellet Heat 30min at 65 Per plug, add 12.5l 10M NH4OAc, 1l glycoblue and 160l of ethanol, vortex well Chill 15 min on ice then spin 15 min top speed 4 Wash with 1 ml 70% ethanol, dry 5 min, resuspend in 130l 1xTE 15min at 65. Note that is 130 l in total, not per plug. Can proceed directly or store at 4 Note: the following sonication protocol is for our Covaries E220, and should yield libraries of 500-600bp final size (including adaptors). If using a different sonicator, you will need to select a sonication scheme that gives average fragment sizes of 400-500bp (which will increase to 500-600bp once all adaptors are added). If you sonicate the library into smaller fragments it is harder to separate the library from the adaptor dimers. The sonication volumes are not too important as the next step is binding to streptavidin beads in 600l of 1x TN. We don t recommend enzymatic fragmentation methods as the ones we have tried chew up the ssDNA bits on the TrAEL-seq adaptor. Transfer to Covaris tubes (AFA Snap-cap microTUBE) Machine use: Turn on machine Turn off and restart! Turn on computer Turn on chiller Fill water tank to 6L Fill in booking sheet Protocol (Cris in JHGroup folder) Duty factor 10% Peak Incident Power 175W Cycles 200 Temp 11C Treatment time 50s Sample volume 130l After use turn everything off, empty water and dry NOTE: From this point one sample = one multiplexed pool (which comes from 1-6 original samples). Do not increase reagent volume for the number of samples in the multiplexed set. Wash 8l Dynabeads MyOne streptavidin C1 beads per sample twice with 0.5ml bead prep buffer (5mM Tris pH8, 0.5mM EDTA, 1M NaCl, 0.05% Tween), 3 min on wheel per wash [you can wash beads for up to 4 samples together] Resuspend beads in 300l 2xTN (10 mM Tris pH 8, 2 M NaCl) per sample, split into 300l aliquots in 1.5ml tubes and add 170l water to each Add 130l sonicated DNA to beads Incubate on wheel 30 min at RT Spin tubes up to 1000rpm before putting on magnet to collect liquid Wash beads 1x 500l 5mM Tris ph8, 0.5mM EDTA, 1M NaCl, 5 min on wheel Spin tubes up to 1000rpm before putting on magnet to collect liquid Wash beads 1x with 500l 0.1x TE, 5min on wheel Spin tubes up to 1000rpm before putting on magnet to collect liquid Carefully remove the wash solution so as not to leave any drops and re-suspend beads immediately in 25 l 0.1x TE Store overnight at 4( or carry on directly Add 3.5l NEBNext Ultra II end repair buffer 1l 1ng/ul salmon sperm DNA 1.5l NEBNext Ultra II end repair enzyme Pipette to mix and incubate 30 min at 20 (room temperature) then 30 min at 65 Let cool then spin 10s at 1000rpm Add 1.25l new ENDseq adaptor 2 (from annealed 10uM stock) 0.5l ligation enhancer 15l NEBNext Ultra II ligation mix Pipette to mix and incubate 30 min at 20 (room temperature) Remove reaction mix using magnet, rinse beads 500ul wash buffer (5mM Tris ph8, 0.5mM EDTA, 1M NaCl). REMEMBER your libraries are on the beads! Wash beads 2x 1ml wash buffer (5mM Tris ph8, 0.5mM EDTA, 1M NaCl) 10 min on wheel each Spin tubes up to 1000rpm before putting on magnet to collect liquid Wash beads 1x with 1ml 0.1x TE, 10min on wheel Spin tubes up to 1000rpm before putting on magnet to collect liquid Carefully remove the wash solution so as not to leave any drops and re-suspend beads immediately in 11l 1x TE + 1.5l USER enzyme Incubate 15 min at 37 This elutes the DNA from the beads Collect beads using magnet, transfer 12l supernatant to a 1.5ml LoBind tube using an orange LoBind tip The supernatant contains the library but we do another round of elution to get anything left. This is the first step in which the very low concentration DNA is unprotected, hence the LoBind tubes and tips Resuspend beads in 10.5l 1x TE + 1.5l USER enzyme Incubate 15 min at 37 This elutes any remaining DNA from the beads Collect beads using magnet, add 12l supernatant to the 12l from the first elution using an orange LoBind tip Store samples at -20 if necessary We then perform a test amplification to work out how many cycles we need to use for the real library. In PCR tubes, mix 1.25l library 0.4l NEBNext any index oligo 0.4l NEBNext universal oligo 2.95ul water 5l NEBNext Ultra II PCR mix 98 30s 98 10s \ 65 75s / x 14 cycles 65 5 min 4 hold Clean with 8l AMPure beads, elute in 2.5l 0.1x TE You can elute up to 2.0l from these but it is easier just to put the eluate and bead mix on the magnet and pipette 1l straight onto the Bioanalyzer lane Run on Bioanalyzer Use this to calculate the cycle number for the final amplification  note: because of the various dilutions, assume that the final library would be 4x more concentrated than the test library, so reduce the number of cycles by 2 before further adjustments. Try to aim for a final library concentration of 1.5-4 nM. Adaptor dimers are often seen in these test reactions, be sure to exclude from the quantification. You should quantify across the region from 300-1,000 bp, which should exclude the adaptor peaks. However, it is not uncommon to get weird profiles like these:  Dont worry your libraries should be fine, but quantification is not so accurate. Err on the side of slightly over amplifying the library (so only reduce the number of cycles by 1 rather than 2 before adjusting the concentration). Library over-amplification doesnt seem to cause big problems in TrAEL-seq. Final library amplification: In PCR tubes, mix 21l library 2l NEBNext index oligo 2l NEBNext universal oligo 25l NEBNext Ultra II PCR mix 98 30s 98 10s \ 65 75s / x calculated cycles 65 5 min 4 hold Transfer libraries to 1.5 ml tubes for final purification (purification seems to be better in 1.5 ml tubes than in PCR tubes), and make sure they are still 50l (top up with water if not). Clean with 40l AMPure beads, elute with 26l 0.1xTE for 25l yield Clean again with 20l AMPure beads, elute with 11l 0.1xTE for 10.5l yield Run 1l directly on Bioanalyser and use 0.2l for KAPA quantification [see separate protocol] It is not unusual to have some residual adaptor in the library. If so, dilute to 25ul and perform another 2 rounds of AMPure bead purification at 0.8x. Important sequencing info: It is critical that at least 10% of some library that is not multiplexed TrAEL-seq be included in the sequencing lane! This is because the invariant T read as the first base after the barcode crashes the base calling algorithm (at least on our NextSeq 500). This causes the surrounding bases in the barcode to be mis-read and not assigned. Spiking in another type of library fixes it. Barcodes for TrAEL-seq index adaptors: We currently have a working set of 6 adaptors - indexes 1, 3, 5, 6, 7, 8 In the adaptorWhat is actually readQuality1GACTagtcGood2AGTCgactPoor - Not in use3CAAGcttgGood4TCGAtcgaPoor - not in use5CCTTaaggGood 6GGAAttccGood7GCACgtgcGood8TGGCgccaGood9CATCgatgMaybe poor - not in use? As an example this is the structure of TrAEL index 1 TrAEL index 1 /5Phos/GACTNNNNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGUAGCA/iBiodT/TGCUACACTCTTTCCCTACACGACGCTCTTCCG Note: the quality of these adaptors synthesised by Sigma-Genosys was poor. We have had all of them synthesised by IDT with very good results. Reagents Adaptors TrAEL-seq index 1: /5Phos/GACTNNNNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGUAGCA/iBiodT/TGCUACACTCTTTCCCTACACGACGCTCTTCCG TrAEL-seq index 3: /5Phos/CAAGNNNNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGUAGCA/iBiodT/TGCUACACTCTTTCCCTACACGACGCTCTTCCG TrAEL-seq index 5: /5Phos/CCTTNNNNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGUAGCA/iBiodT/TGCUACACTCTTTCCCTACACGACGCTCTTCCG TrAEL-seq index 6: /5Phos/GGAANNNNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGUAGCA/iBiodT/TGCUACACTCTTTCCCTACACGACGCTCTTCCG TrAEL-seq index 7: /5Phos/GCACNNNNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGUAGCA/iBiodT/TGCUACACTCTTTCCCTACACGACGCTCTTCCG TrAEL-seq index 8: /5Phos/TGGCNNNNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGUAGCA/iBiodT/TGCUACACTCTTTCCCTACACGACGCTCTTCCG Purchase PAGE purified from IDT These must be 5 adenylated (5 App) before use using the 5 adenylation kit (see separate protocol for this) We strongly advise testing that your set of adaptors works efficiently before using in TrAEL-seq as we have repeatedly had problems. We have provided a separate protocol and python script for testing the adaptors and analysing the results. TrAEL-seq adaptor 2 (aka: ENDseq adaptor 2): [Phos]GATCGGAAGAGCACACGTCTGAACTCCAGTCUUUUGACTGGAGTTCAGACGTGTGCTCTTCCGATC*T Purchase PAGE purified from IDT Annealing: 20l 100uM ENDseq Adaptor 2 in 200ul 1x T4 DNA ligase buffer Incubate in heating block at 95C 5 min then remove block from the heat and leave to cool to room temperature Reagents and consumables Terminal Transferase (NEB M0315S) 100mM ATP (Merck) aliquot as 5ul in PCR tubes on receipt and store at -80 T4 RNA ligase 2 truncated KQ (NEB M0373L) T4 RNA ligase buffer (NEB supplied with T4 RNA ligase 2) 50% PEG 8000 (NEB supplied with T4 RNA ligase 2 or home-made as required) -agarase (NEB M0392S) GlycoBlue (Thermo AM9515) 1l Bst 2 WarmStart DNA polymerase (NEB M0538S) Isothermal amplification buffer (NEB supplied with Bst pol) 100 mM MgSO4 (NEB supplied with Bst pol) 10 mM dNTPs Ethanol AFA microTUBEs (Covaris 520045) Dynabeads MyOne streptavidin C1 beads (Thermo, 65001) NEBNext Ultra II DNA kit (NEB E7645S), additional Q5 mix may be required which can be purchased separately from NEB 1ng/l sonicated salmon sperm DNA or other carrier NEBNext Multiplex Oligos set (e.g. NEB E7335S), additional USER mix may be required which can be purchased separately from NEB AMPure XP beads (Beckman A63881) Bioanalyser high sensitivity DNA chip (Agilent 5067-4626) KAPA qPCR (Roche KK4835) or equivalent library quantification kit Solutions These solutions are made with milliQ water Tris buffer 10mM Tris pH8.0 Agarase buffer 10 mM Bis-Tris-HCl, 1 mM EDTA pH 6.5 These solutions are made with certified DNase/RNase free water 10 M NH4OAc 1x TE and 0.1x TE Diluted from 100x stock 2xTN 10 mM Tris pH 8, 2 M NaCl Wash buffer 5 mM Tris pH 8, 0.5 mM EDTA, 1 M NaCl Bead prep buffer 5mM Tris pH8, 0.5mM EDTA, 1M NaCl, 0.05% Tween Equipment Magnetic rack for 1.5 ml tubes Magnetic rack for 0.2 ml tubes (optional) Covaris E220 sonicator Other sonicators may be suitable, protocol adjustments will be required PCR machine Agilent Bioanalyzer or equivalent gel system Wheel Rocker 2 heating blocks LoBind 1.5ml tubes LoBind tips for P20 Common TrAEL-seq problems and solutions Cell number For yeast, samples of <1x106 cells perform poorly, 1x107 seems optimal. 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Data can be rescued by trimming 3nt from the start of the dead, but assigning indexes becomes very difficult. Fragmentation TrAEL-seq libraries should be fragmented to fairly large fragments such that the final average library size is 600-800nt. Much smaller and the libraries are hard to separate from the often large amount of adaptor dimers produced. Other sonication systems should be effective, but some chemical fragmentation systems will cleave single stranded regions of TrAEL-seq adaptors. Test amplification This can often yield strange bioanalyser profiles. Dont worry, final libraries are usually fine and quantitation based on the 200-1000bp region remains fairly accurate. Excessive adaptor diners Although most libraries amplify well, some have problems particularly if the input amount is low. We have noted that performing a 0.8x AMPure cleanup after USER elution prior to PCR can dramatically reduce adaptor dimers. Where we have heard of others having this problem, it has been through sonicating fragments to a smaller size then gel extracting to separate from adaptors. We dont know why this is an issue but it does seem to cause problems. Yield and purity We originally suggested aiming for 1nM final library concentration, but experience shows this to be a bit low and we now aim for 2-4nM. This allows libraries to be further AMPure cleaned at 0.8x to remove residual adaptor dimers (not uncommon). Additional PCR cycles can be performed if final library concentration is low, but a further two rounds of AMPure cleanup are required afterwards. Choice of multiplex sets Differences in TrAEL-seq substrate amounts will lead to differences in the read output across multiplexed sets. Some cell lines give particularly strong TrAEL-seq signals (we have seen this with SW620 for example), and will tend to swamp other samples multiplexed in the same pool such that the majority of reads stem from a few libraries while others are under-sequenced. We therefore recommend not mixing cell lines within a pool, and if detailed analysis of a poorly replicating cell type is required (eg: some drug treated samples), it is wise not to mix these with untreated controls.  Ethanol fixing of mammalian cells yet to be tested  Stored in 5ul aliquots in the -80 from a Merck stock solution  Use home-made 50% PEG the kit does not come with enough  Care! Not T4 DNA ligase buffer!  Home made, but same as NEB agarase buffer  Make sure these really reach temperature before use! Give them at least 20 min  This is a recent modification. Almost all multiplexed libraries have been made by pooling up to 3 plugs in a tube and adding 50ul per plug of agarase buffer. Two tubes, each containing 3 plugs, have then been combined after precipitation but before the Bst step. This modification does not seem to make a difference and is simpler.  Sample didnt pellet and formed a weird gelatinous lump? Weve seen this with HeLa samples. We added some 3M NaOAc and some more ethanol and re-span, that sorted the problem.  Dont use beyond the expiry date you get lots in a tube but this enzyme can go off and that causes subtle but significant problems  With our current filter tips it is very hard to remove material from Covaris tubes use a normal yellow tip instead  This is included to buffer the voracious activity of T4 DNA polymerase in the End repair mix so it doesnt degrade rare DNA fragments. It may not be necessary.  Note do NOT use the ENDseq 2c adaptor it will not work!  This is for 4-6 multiplexed mammalian samples, use 15 cycles for 2-3 samples or 16 cycles for 1 sample. Increase by 1 for yeast samples or if you have reason to believe your signals will be low. It is not critical you get this right, you can always do another test PCR if your amplification is wildly under or over. The whole point is to be able to calculate the perfect cycle number for the final amplification.      FILENAME TrAEL-seq.doc v2.3 Houseley lab  PAGE 4 56uvPQPQlmqrNO./ĺĶĶĶĶĞ~~zrnnrnnhbjhbUh7hVgjhVg0JUhjh0JUh/djh/d0JUhptjhpt0JUhk"9jhk"90JUh1jh10JUhAD8jhAD80JUhFjhF0JUhjh0JUhi+PPN.gdrgd1 绵禟 hdThh7hio0JmHnHu h(0Jjh(0JUh%h hOThGdXh(mHnHuhvmHnHuh(jh(UhbjhbU,1h. 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